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KMID : 1004820110120040215
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Journal of Biomedical Research
2011 Volume.12 No. 4 p.215 ~ p.223
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Treatment with Bisphenol A Leads to the Promotion of Human Breast Cancer Cells and Alteration of Cell Cycle-Related Gene Expressions, cyclin E and p27
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Lee Hye-Rim
Kim Tae-Hee
Choi Kyung-Chul
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Abstract
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Endocrine disrupting chemicals (EDCs) have detrimental effects on human health. Among these EDCs, bisphenol A (BPA) binds to estrogen receptors (ERs) to stimulate estrogen-mediated responses. BPA is assumed to disrupt the reproductive and developmental system of humans. In addition, BPA has recently been suspected as a risk of carcinogenesis. Because BPA can cause abnormal estrogen-mediated response in the organism, exposure to BPA may stimulate growth of estrogen-dependent breast cancers in human. In breast cancer, cyclin E and cyclin-dependent kinase inhibitor p27 are important in G1/S phase transition during cell cycle progression. In this study, using an MTT assay, we investigated the effect of BPA on proliferation of MCF-7 breast cancer cells in vitro. In addition, we also analyzed the transcriptional levels of cyclin E and p27 following treatment with BPA using semi-quantitative RT-PCR. As a result, treatment with BPA resulted in significant induction of breast cancer cell growth, compared to a vehicle. BPA caused alterations of cyclin E and p27 mRNA expression. Expression of cyclin E was increased by BPA, while p27 was decreased at 24 h after treatment with BPA in MCF-7 breast cancer cells. Taken together, these collective results suggest that exposure to BPA induced breast cancer cell proliferation with deregulation of the cell cycle. A further study is required in order to determine the effects of BPA on the carcinogenic process in in vivo models.
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KEYWORD
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Endocrine disruption, Bisphenol A, Human breast cancer, Cyclin E, p27
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